Cuvette challenge
Prepare the sample
Predicted absorbance
0.000
Predicted transmittance
100%
Concept: More absorbing molecules along the light path mean less transmitted light.
The maths
A = εclT = 10−A%T = 100TA = 2 − log₁₀(%T)c = A/(εl)ε: L/(mol·cm); c: mol/L; l: cm; A: dimensionless; %T: percent.
Assumption: homogeneous, non-scattering solution at one wavelength, in the linear Beer–Lambert regime.
Live quantitative plots
Calibration and transmission
Model curve
Current sample ●
Experiment controls